Gateway invitrogen manual

 · Here is an archived copy of the Invitrogen 3-insert multisite Gateway manual, version D, We originally had a live link to the Invitrogen site, but after this link broke it seemed easier to hard-archive a copy. TOPO TA Cloning—To create a Gateway entry clone. Step 1—Produce PCR product. Produce PCR products using Taq polymerase and your own protocol. End the PCR reaction with a final 7 to 30 minute extension step. Step 2—Perform the TOPO Cloning Reaction. Set up one of the following Invitrogen TOPO Cloning reactions using the reagents in the. specialized or customized vector, the Invitrogen™ Gateway™ Vector Conversion System can convert any vector into one compatible for Gateway cloning. Figure 1. Gateway technology facilitates cloning of genes into and back out of multiple vectors via site-specific recombination. Once a gene is cloned into an Entry clone, you can thenFile Size: KB.

Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent. E. coli. Efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System. Manufacturer: Invitrogen™ K User Manual Corporate Headquarters Invitrogen Corporation Faraday Avenue Carlsbad, CA T: 1 7F: 1 7E: www.doorway.rue@www.doorway.ru For country-specific contact information visit our web site at www.doorway.ru MultiSite Gateway® Pro Using Gateway® Technology to simultaneously clone multiple DNA fragments. User Manual Corporate Headquarters Invitrogen Corporation Faraday Avenue Carlsbad, CA T: 1 7 into a vector for entry into the Gateway ® System or the MultiSite Gateway System available from Invitrogen. Blunt-end PCR products clone directionally at greater than 90% efficiency, with no ligase, post-PCR procedures, or.

Here is an archived copy of the Invitrogen 3-insert multisite Gateway manual, version D, We originally had a live link to the Invitrogen site, but after this link broke it seemed easier to hard-archive a copy. TOPO TA Cloning—To create a Gateway entry clone. Step 1—Produce PCR product. Produce PCR products using Taq polymerase and your own protocol. End the PCR reaction with a final 7 to 30 minute extension step. Step 2—Perform the TOPO Cloning Reaction. Set up one of the following Invitrogen TOPO Cloning reactions using the reagents in the. specialized or customized vector, the Invitrogen™ Gateway™ Vector Conversion System can convert any vector into one compatible for Gateway cloning. Figure 1. Gateway technology facilitates cloning of genes into and back out of multiple vectors via site-specific recombination. Once a gene is cloned into an Entry clone, you can then.